With a wavelength range from 340 to 850 nm, it is the equivalent to having 510 free filters. Readings were taken every 15 s at 490 nm and the results were analysed using SOFT-MaxPro 4. 45% of MTT reagent in cell culture media was substituted and incubated for 4 h before adding 100 μL of 100× DMSO. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. To measure the luciferase activity biochemically, cells wereThe activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for its absorbance, luminescence,. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. 3 Methods 1. Access all the resources, tools and information you need to stay productive. FAST AND ACCURATE METERTECH Microplate Reader $3,650. The reaction was stopped with 100 µL of H 2 SO 4 1 M and quantified in a spectrophotometer (VERSAmax, Molecular Devices, San Jose, CA, USA) at 450 nm. Printed in U. 0A-Sunnyvale, CA-Made in USASpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Get pricing and purchase lab equipment and. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmSpectraMax and VersaMax . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. Add to Cart. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). 17. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). Select Start>Control panel>Device Manager. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochrome-based systems. 5 mM EDTA, 0. The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). 0 nm full width half maximum. Global research efforts are focused on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including: vaccines. We would like to show you a description here but the site won’t allow us. The limit of detection for each assay was 156. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. The sections were stained with hematoxylin and eosin (H&E) and. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The average OD 490 of 12 blank wells (Line 18) was then subtracted from the raw OD 490 obtained for each sample well. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. S. All. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. TECHNICAL NOTE. 0A-Sunnyvale, CA-Made in USA SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Values are presented as the mean ?standard deviation (SD). Supplier: Molecular Devices. Manufacturer: Molecular Devices Model: VERSA MAX Turnable Microplate reader Further Information Descriptions, data, dimensions and quantities are based on the subjective beliefs of the auctioneer and are created to the best of our knowledge and belief. Absorbance plate reader (e. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The nuclear extracts were subsequently determined for the content of NF-κB with the NF-κB (p65) transcription factor assay kit. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. The absorbance was measured at 450 nm with 650 nm as a reference on a VersaMax (Molecular Devices, Sunnyvale, CA) and a standard curve was plotted using a 4-parametric mathematical fit model in. Microplate Reader Shelf. , Sunnyvale, USA). VersaMax™ Tunable Microplate Reader, Molecular Devices. 1 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 compliance to ensure data integrity. After addition of stop solution, the optical density of samples were read at wavelengths of 450 and 570 nm (Versamax, Molecular Devices Co. 2 and 648. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate. Primer design. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The number of cells that migrated to the bottom was determined by measuring the optical density at 590 nm, using a microplate reader (VersaMax; Molecular Devices). For more information, please visit us at With the VersaMax microplate reader, you will never have to buy another filter. The VersaMax measures thMolecular Devices memberdayakan para ilmuwan untuk memajukan diagnosis dini dan terapi yang lebih aman untuk pasien. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. Used. End-point IgG titers were established as the mean plus three standard deviations of. MOLECULAR DEVICES VersaMax is a customizable lab equipment capable of performing multiple types of research-grade tests and experiments at once, with enhanced safety protocols and adjustable column and sample-loading system for efficient sample preparation and increased throughput. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. $1,749. Briefly, MTT (200 μl, 2 mg/ml) was added to the wells and incubated for 4 h at 37°C. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. Lihat Video. to 11. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. SoftMax® Pro, the industry-leading microplate reader software, is included at no. Product Overview VersaMax™ ELISA Microplate Reader Manufacturer Molecular Devices® | Available Worldwide Be the first to review this product Request Pricing Molecular Devices® Manufacturer Website. Cell viabilities were presented as the ratio of the absorbance of treated cells to the absorbance of control cells cultured with an extract of HDPE. Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. Used. An aliquot of the extracts dissolved in DMSO with 80 μL of phosphate buffer (pH 6. Tristar 3 Multimode Microplate Reader. SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. $1,195. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. If the computer is connected to the Internet: Select Search Automatically for updated driver software. 1. The cytokine concentration was calculated from the standard curve using seven-parameter curve fitting software (SOFTmaxPro 5. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Sterile AB medium and a mixture of Me 2 SO, methanol, and water (ratio v/v 1:1:8) served as negative controls. Find regional support. 003 OD ±1. Molecular Devices VersaMax Microplate Reader. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Get pricing and purchase lab equipment and. Conventions Used in this Manual. The average survival rates for cells exposed to dry. This reliable model allows for. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). , St. . The VersaMax Absorbance Microplate Reader allows for selection of. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. ChE activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction. Nude mice were obtained from Orient Bio Inc. 747. 4. MOLECULAR DEVICES VMAX KINETIC MICROPLATE READER. 2. com we know that an user's manual may be needed at any time. 00 Add to cart Molecular Devices Microplate Reader Spectramax 384 340PC $ 6,295. After that, absornace were measured at 520 nm using using a microplate reader spectrophotometer VERSAmax (Molecular Devices). The. 4. (VersaMax, Molecular Devices, Sunnyvale, CA, US). 5 mM was used to make the. the plate reader (model VERSAmax, Molecular Devices, USA) at 515 nm (A515). The in vitro differentiation of BMDMs. ® Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. 제조사 : Molecular Devices. Reverse transcription-quantitative polymerase chain reaction To assess phospholipase C gamma 1 (PLCG1) and phospholi-pase C gamma 2 (PLCG2) mRNA levels in each. Molecular Devices Versamax; Molecular Devices Vmax; Molecular Devices Fmax Fluorescence; Packard Fluorocount Fluorescence; Packard Spectracount; Tecan Spectrafluor Plus Multilabel; Perkin Elmer HTS-7000 Plus Multilabel; Skatron Skanstacker 300 Washer; Berthold LB96P Luminescence;Finally, cells were incubated with 1% SDS for 2 h at RT to release the dye from the cells, and the cell proliferation rate was assessed by quantification of the amount of dye released from the cells using a spectrophotometer (VersaMax, Molecular Devices) at the wavelength of 570 nm. (VersaMax, Molecular Devices, USA). DISCLAIMER Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Turn the reader on and secure the USB cable. 5 × 10 6 cells per well inoculated into 6-well plates in culture medium with CSF1 (10 ng/mL) for 7. Measurement of brain-derived neurotrophic factor (BDNF) release. The absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. Windows Home Editions are not supported. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and. To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. 1 M sodium phosphate buffer (pH 6. HL-60 cells were plated at a density of 3×105 cells/well on 6-well plates. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. Preconfigured method protocols specifically written for the Pyrochrome Assay and other ACC assays are available for SoftMax Pro Software and take. 1 software, the leading microplate data acquisition and analysis software, you can download this PDF file from Molecular Devices. 747. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. , Sunnyvale, CA, USA). ). Find regional support. The lysozyme concentration of a sample was determined using a standard calibration curve made of a series of known lysozyme concentrations. 0 OD Kinetic Read Time: 9 Second Minimum Interval Temperature Uniformity ±0. Molecular Devices Corporation. For wells that were not exposed to dehydration (positive control), all the cells (100%) survived for the 60-min test. SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. 4. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 1. ChE activity was normalized by protein concentration as determined by a Coomassie. $199. # SMP54-GXP- 10UL) Pyrochrome Assay. $1,195. Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All. 00 Regular price. Used. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 6/10 #0120-1330d. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. And the VERSAmax reader is robotics-compatible. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 5 mg/mL) in the range of 5–100 μg per 0. BMG Labtech - THERMOstar. As a result, assay flexibility is consequence-free. MiR-21. Spectranet customer portal. Thus, the molecular mechanism promoting hepatic lipid accumulation in obesity is of biomedical and public health interest. Then, the cultured cells were treated with different. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. For determination of the optimal temperature, EfmTDC was incubated in 100 mM sodium acetate buffer (pH 5. 47 shipping. 0 out of 5 $ 23,995 $ 22,495. Microwell plates, black (96-well) Rocking table with adjustable lifting frequency. Cells were treated with 150 μl of dimethylsulfoxide (DMSO) and absorbance was quantified at 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). These values were transformed to logarithmic scale to create a log-log curve. The optical density (OD) was measured at 570 nm (with a reference at 690 nm) by UV-visible spectrophotometer (VersaMax, Molecular Devices, CA, USA) in triplicates. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard. At 48 h after transfection the cells were exposed to CoCl 2 (200 µM) or vehicle. Figure S2: The cellular localization of NDRG1 is not affected by N- or C- terminal deleted NDRG1 mutants under hypoxic conditions. Cell migration assay. StakMax Microplate Handling System. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. FRAP solutions were prepared as described previously (Benzie and Strain, 1996, Benzie and Strain, 1999). 00 USD. 2 mM PLP and 5. absorbance readers. SpectraTest ABS1 Absorbance Validation Plate. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. printed in u. After treatment, cells were collected, lysed with the lysis buffer. MDC instruments including: SpectraMax®, VersaMax. 00. Cell cycle investigation. The absorbance of the treated and untreated samples at 450 nm was then measured on a multi-well microplate reader (VersaMax, Molecular devices, San Jose, CA, USA). After three times washing, the reaction was colored by substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (T0440, Sigma), and stopped by 2 N HCl. A microplate reader (VersaMax, Molecular Devices, USA) was used to detect the OD value at 450 nm. Published Dec 16, 2020 | Updated May 19, 2023. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Bacterial growth was determined turbimetrically using the microplate ELISA reader VERSAmax (Molecular Devices). The color was allowed to develop for 20 min in dark and the reaction was stopped with 2. This robust model allows precise wavelength detection in. Furthermore, VersaMax is reliable, which means more uptime and lower long-term cost of. Sing a Microplate Reader (VersaMAx, Molecular Devices). To test if sodium arsenite and dimethylarsenic acid could stimulate apoptosis in 3 cells, oc cell cycle redistribution was studied using flow cytometry with propidium iodide staining. Also see: SoftMax® Pro Microplate Software User Manual Download Page. 0 out of 5 $ 2,999. The signal was developed using 3,3′, 5,5′-tetramethylbenzidine after streptavidin–peroxidase treatment. Flow Cytometry AnalysisAt the end of the exposure, the MTS solution (20 µL/well) was added, and the cells were incubated for 3 h at 37°C. [Google. ~ Bio-Tek ELx808 Microplate Reader $2,795. Sale Sold out. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 00 USD Tecan Infinite F200 Reader Fluorescence Reader w WARRANTY $2,750. 3 Methods 1. Cell viability was expressed as percent survival of untreated cells. Our specialization servicing microplate instrumentation and our focus on a narrow range of product lines allows us to provide the highest standards of service and ensure. We would like to show you a description here but the site won’t allow us. New and used Molecular Devices VersaMax's for sale on LabX. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). ,, [28]. All analyses were performed three times, and the mean values were used for further calculations. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software,Get the most out of SoftMax Pro. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 5×10 3 cells were seeded in 96-well plates, treated with indicated inhibitors for 48 or 72 hours, analyzed 16 hours after BrdU addition, and absorbance measured at 450nm using a spectrophotometer plate reader (VersaMax, Molecular. ®SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Reboot the computer. , Sunnyvale, CA). The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Glossary of Terms. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. StakMax. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. microtiter plate reader (VERSAmax, Molecular Devices). 00 USDLocation: Hampton, New Hampshire, United States Member Since: 28th February, 2012 (12 years ago) Current Listings: 3982 Return Policy: WE OFFER 180 DAY WARRANTY ON ALL ITEMS. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The emodin thermoreversible PG was. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can. And the VERSAmax reader is robotics-compatible. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. ® US HEADQUARTERS. Table of Contents. Add to Cart. Our absorbance plate readers feature our PathCheck Sensor technology and industry-leading SoftMax® Pro Data Acquisition and Analysis Software. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Versamax Microplate Reader dibangun di sekitar monokromator kisi. Western Blot. 9 in. Select Start>Control panel>Device Manager. 0A Sunnyvale, CA Made in USAVersaMax price. Results of host range test of Mae-Yong924-1 against. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. $199. Absorbance was determined at 490 nm on a microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). 2. 00 Add to cart Molecular Devices Microplate Reader Spectramax 384 340PC $ 6,295. Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机，拥有灵活检 测优势，无需加配额外的滤光片。 可调波长，无需滤光片，灵活 VersaMax读板机使用光栅单色器来选择 波长，其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到 With the VersaMax microplate reader, you will never have to buy another filter. Spectrophotometers. Molecular Devices also offers a mass spectrometry-based solution for food safety testing We would like to show you a description here but the site won’t allow us. reader (VersaMax, Molecular devices, San Jose, CA, USA). Assays were conducted in a 96-well microplate using ELISA microplate reader (VersaMax Molecular Devices, USA) and absorbance was measured at 475 nm. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. S. Cell-free AB broth (22) of an overnight culture of the acylhomoserine lactone-defective V. Accession codes. SoftMax Pro. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Spectrophotometers. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. This. The culture supernatant of cells (25 μl) was mixed with 225 μl of DMMB dye solution and the absorbance of solution mixture was measured at 525 nm with a spectorophotometer (VERSAmax; Molecular Devices Japan KK, Tokyo, Japan). The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 8. molecular devices | 1311 orleans drive | sunnyvale, cA 94089 usA | email: info@moldev. The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). 00 Sale price $ 0. 00 Sale price $ 0. 99 + $91. DNA content in the supernatant (100 μl) was measured using a Quant-iT PicoGreen assay kit (Invitrogen) as per the manufacturer's instructions. Find regional support. 1 mL. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Enzyme activity was. The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). Select Update Driver. Softmax Pro 소프트웨어가 포함된 PC 워크스테이션 포함. Molecular Devices GMBH Tel. Innovative and flexible design features enable accelerated sample preparation time and increased laboratory productivity of DNA, RNA, and protein samples. The absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 450 nm. While the cell covers about. Open the Windows Task Manager, go to the Services tab, and click Open Services. Lihat Video. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. 00. The absorbance of the mixture was measured with a microplate reader (VERSAmax™, Molecular device, Sunnyvale, USA) at wavelength of 498 nm until absorbance was saturated. We would like to show you a description here but the site won’t allow us. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. One microgram of RNA was. 파장 범위(nm) : 340–850. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein. Tunable, filterless flexibility The. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. The value for non-specific absorption at 600 nm was subtracted. 72 MB. 03 billion doses of. Select Add or Remove Programs system settings. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. It features a modular design that allows users to add or change detection cartridges according to their needs. VersaMax ELISA Microplate Reader. from Molecular Devices Corporation. Table 1. The absorbance was measured at 490 nm using a VersaMax ELISA reader (Molecular Devices, Sunnyvale, California, United States). ®ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 00. SpectraTest® Validation Package. A. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. All samples were measured in triplicate. S. Software (optional), such as Softmax Pro (Molecular Devices). +81-6-6399-8211 fax +81-6-6399-8212 Nihon Molecular Devices Tokyo Tel. Concluding Remarks. At All-usermanuals. Molecular Devices Corporation. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax microplate spectrophotometer provides rapid and sensitive measurements for a variety of analytes across a wide range of concentrations. Cell proliferation assay MDA-MB-231 cells were seeded at a density. The absorbance was measured at 405 nm using a plate reader (VersaMax, Molecular Devices) (N = 3). 8), 40 μL of tyrosinase, and 40 μL of L-DOPA was put in each well. All animal experiments were performed in compliance with the relevant laws and. The narrowly focused light beam and optical design give you the same high performance with round bottom, flat bottom, or half area well plates. 4 Microplate Reader Software w WARRANTY. 17. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. Microplate Reader Shelf. Lipid accumulation was assessed by ORO staining. 0 out of 5 $ 2,999. g. The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). 635. Cells were lysed in NETN buffer (100 mM NaCl, 20 mM Tris pH 8. Click SoftMax Pro Software User Guide. Flexible calculations Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. We would like to show you a description here but the site won’t allow us. Turn the reader on and secure the USB cable. Cell viability was analyzed using a cell counting kit assay (Dojindo). together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. The system provides automation for up to 50 For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The wells were visualized using o-phenylenediamine (OPD), and the absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 490 nm. Table of Contents. The signal produced was proportional to the amount of analyte present and interpolated from the calibration curve presented on each plate. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). Use Windows Search to search for “Add or Remove”. This manual does not represent a commitment on the part of Molecular Devices Corporation. New and used Molecular Devices VersaMax's for sale on LabX. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. The Indocyanine green (ICG, Sigma-Aldrich) assay measures the liver depuration capacity .